Figure 4: TREM-1 influences the composition of aortic myeloid cell subsets.

(a–e) Flow cytometry analysis of aortic wall-infiltrating myeloid cell subsets isolated from individually digested aortas of 16-week HFCD-fed Trem1^+/+ Apoe^−/− and Trem1^−/− Apoe^−/− mice. (a) Representative gating strategy for identification of myeloid cell subsets within single, live, non-autofluorescent CD45⁺, CD11b⁺ non-dendritic cells (Trem1^+/+ Apoe^−/−). Identity of neutrophils was confirmed by inclusion of Ly6G in the staining panel. (b) Representative staining panels for expression of the monocyte/macrophage marker CD64 (line) on the indicated cell subsets (filled histograms: matched isotype control-stained cells) (Trem1^+/+ Apoe^−/−). (c) Representative staining panels for expression of surface TREM-1 (line) on the indicated cell subsets (filled histograms: matched isotype control-stained cells) (Trem1^+/+ Apoe^−/−). (d) MFI values for TREM-1 surface expression (with subtracted MFI values of matched isotype control-stained cells) on the indicated myeloid cell subsets of Trem1^+/+ Apoe^−/− mice. Circles represent data for individual mice, lines indicate mean values per group. (e) Relative abundance (% among CD45⁺ cells) of arterial wall-infiltrating cell subsets in 16-week HFCD-fed Trem1^+/+ Apoe^−/− versus Trem1^−/− Apoe^−/− mice. Circles represent data for individual mice from three independent pooled experiments, lines indicate mean values per group. *P<0.05 as determined by the two-tailed t-test. Statistically not significant differences with P>0.05 are not indicated.