Figure 5: TREM-1 synergizes with dyslipidemic factors for pro-inflammatory cytokine secretion.
From: TREM-1 links dyslipidemia to inflammation and lipid deposition in atherosclerosis
(a–c) Human CD14hi monocytes were FACS-purified from buffy coats obtained from different blood donors. (a) Representative histograms for TREM-1 surface expression (lines; filled histograms represent isotype control stained cells) on human peripheral blood CD14hi monocytes after 20 h of stimulation with the indicated stimuli. (b) MFI values for TREM-1 surface expression after subtraction of MFI values for matched isotype control-stained cells. Bars show mean values of seven independent experiments with error bars indicating the s.d. (c) Production of IL-1α, IL-1β and TNFα by CD14hi monocytes after 20 h of culture with the indicated factors. Symbols show individual values of 6–7 independent experiments and different blood donors. *P<0.05, **P<0.01, ****P<0.0001 as determined by the one-way (b) or two-way (c) ANOVA test. Statistically not significant differences with P>0.05 are not indicated.
