Figure 3: Class IIa HDACs are required for PTH-induced SOST suppression in vivo.
From: SIKs control osteocyte responses to parathyroid hormone
(a,b) 6 week old male mice of the indicated genotype were treated with vehicle or PTH (1-34, 300 μg kg−1) and euthanized 90 min later. Bone RNA was obtained and RANKL and SOST transcript abundance was determined by RT-qPCR. * indicates P<0.05 comparing vehicle and PTH for each genotype. N=at least 6 mice per group were analysed. (c) Representative photomicrographs of sclerostin immunohistochemistry from WT and DKO mice treated with vehicle or PTH. Scale bar, 40 μm. (d) Quantification of immunohistochemistry results. Cortical osteocytes in a fixed region of bone 3 mm below the tibial growth plate were counted and scored as either sclerostin-positive or negative. N=6 mice per group were analyzed. * indicates P<0.01 comparing vehicle and PTH for each genotype by student’s unpaired two tailed t-test.
