Figure 6: YKL-05-093 and PTH similarly affect gene expression.

(a) Venn diagram showing overlap between differentially-expressed genes (fold chance >2, FDR<0.05) determined by RNA-Seq from Ocy454 cells treated with vehicle, PTH (1 nM) or YKL-05-093 (0.5 μM) for 4 h. (b) Heat map showing six different clusters of differentially expressed genes. Each row corresponds to a single differentially expressed gene. Colour coding is with respect to the average log2 (fold change) for each gene comparing treatment to vehicle. Genes were ordered by the strength of the significance of the fold change comparing PTH and vehicle. (c–h) Ocy454 cells were treated with vehicle, PTH (1 nM) and YKL-05-093 (0.5 μM) for 4 h, and RT-qPCR was performed for the indicated gene. As described in the text, FAM69C and KLHL30 are regulated by PTH alone, ADAMTS1 and DUSP6 are regulated by YKL-05-093 alone, and WNT4 and CD200 are regulated by both PTH and YKL-05-093. (i,j) Control and SIK2/3-deficient cells were treated with vehicle or YKL-05-093 (0.5 μM) for 4 h, and WNT4 and CD200 transcript abundance determined by RT-qPCR. For all panels, * indicates P<0.05 comparing vehicle and compound or PTH treatment by student’s unpaired two tailed t-test.