Figure 2: HIF-dependent MYC and PVT1 induction in ccRCC. | Nature Communications

Figure 2: HIF-dependent MYC and PVT1 induction in ccRCC.

From: Genetic variation at the 8q24.21 renal cancer susceptibility locus affects HIF binding to a MYC enhancer

Figure 2

(a) HIF-1β ChIP-seq signals at the 8q24.21 renal cancer susceptibility locus in non-renal cells (green) or renal tubule-derived cells (HK-2, HKC-8: immortalized tubular cells, PTC: primary tubular cells, ccRCC: 786-O and RCC4, blue). In non-ccRCC cells, HIF was stabilized by hypoxia or DMOG for 16 h. rs35252396 is located close to the HIF peak (highlighted in light blue) ∼14 kb upstream of the transcriptional start site of PVT1. (b) ChIP-seq (H3K4me1 and H3K27ac), DNaseI-seq and FAIRE-seq signals in MCF-7 breast cancer and 786-O renal cell carcinoma cells reveal features of active and open chromatin at the 8q24.21 SNP-associated HIF-binding site (highlighted in light blue) in 786-O cells, but not MCF-7 cells.

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