Figure 2: mTOREKO mutants are viable but fail to form a stratified and protective epidermis.
From: mTORC1 and mTORC2 regulate skin morphogenesis and epidermal barrier formation
(a) Representative H&E-stained back skin in newborns. (b) Quantification of hair follicles (HF) on back skin (n=5 mice/genotype). (c) Representative H&E-stained tongue of newborns. (d) Representative immunostaining of epidermal markers: keratin 14 (K14, green), keratin 10 (K10, red), keratin 15 (K15, red), filaggrin (red) and loricrin (red) of back skin in newborns, (DAPI stain, blue), dashed line indicates basal membrane. (e) Toluidine blue dye penetration assay with newborn mice. (f) Perinatal weight loss of newborns. e, epidermis; d, dermis; hf, hair follicle; m, muscle; scale bar, (a,c) 50 μm, (d) 25 μm. Data represents mean±s.d.; non-paired t-test was used to calculate P value. **P<0.01.
