Figure 3: mTOREKO embryos fail to initiate epidermal stratification.
From: mTORC1 and mTORC2 regulate skin morphogenesis and epidermal barrier formation
(a) Macroscopic appearances of mTOREKO and control embryos. (b) Toluidine blue dye penetration assay and the quantification of the blue stained area (n=5 embryos/genotype). (c) Representative H&E-stained back skin of embryos and the quantification of epidermal thickness at indicated time points (n=5 embryos/genotype). (d) qRT–PCR analysis and representative immunostaining of keratin 8/18 (K8/K18) in mTOREKO and control epidermis at E17.5 (n=5 embryos/genotype). (e) BrdU-immunostaing and quantification of basal BrdU+ cells in embryo epidermis at E16.5 and E17.5 (n=5 embryos/genotype). (f) Representative TEM of epidermis at E15.5 reveals an atypical epithelial cell morphology of basal cells with loss of the typical cubical morphology recognizable in E15.5 control epithelium (n=3 embryos/genotype). Scale bar, (c–e) 25 μm, (f) 2 μm. Data represents mean±s.d.; non-paired t-test was used to calculate P value. *P<0.05, **P<0.01, ***P<0.001.
