Figure 5: Epidermal-specific deletion of Rptor phenocopies mTOREKO mice.
From: mTORC1 and mTORC2 regulate skin morphogenesis and epidermal barrier formation
(a) Representative western blotting analysis for Raptor protein in epidermis isolated from control and RapEKO newborns. (b) Macroscopic appearance and (c) Toluidine blue dye penetration assays with newborn mice. (d) Representative H&E-stained back skin in newborns; quantification of hair follicles (HF) on back skin (n=5 mice/genotype). (e) Representative immunostaining of epidermal markers: keratin 14 (K14, green), keratin 10 (K10, red), keratin 15 (K15, red), filaggrin (red) and loricrin (red) of back skin in newborns, (DAPI stain, blue), dashed line indicates basal membrane; scale bar, (d) 50 μm, (e) 25 μm. Data represents mean±s.d.; non-paired t-test was used to calculate P value. **P<0.01.
