Figure 7: mTORC2 controls stratification and epidermal barrier formation of the interfollicular epidermis.
From: mTORC1 and mTORC2 regulate skin morphogenesis and epidermal barrier formation
(a) Western blot analysis for Rictor protein in epidermis isolated from control and RicEKO embryos. (b) Representative macroscopic appearance of newborns; note fine-wrinkled texture in RicEKO mice. (c) Representative H&E-stained back skin in newborns. (d) Quantification of epidermal thickness and hair follicles (HF) on back skin (each dot represents one mouse). (e) Toluidine blue dye penetration assays with control and RicEKO newborn mice; arrowheads indicate skin lesions and penetration of dye. (f) Quantification of TEWL in newborns (each dot represents one mouse). (g) Representative immunostaining of epidermal markers: keratin 14 (K14, green), keratin 10 (K10, red), keratin 15 (K15, red), filaggrin (red) and loricrin (red) of back skin in newborns, (DAPI stain, blue), dashed line indicates basal membrane. e, epidermis; d, dermis; HF, hair follicle; m, muscle; scale bar: (b) 2 mm, (c) 50 μm, (g) 25 μm. Data represents mean±s.d.; non-paired t-test was used to calculate P value. ***P<0.001.
