Figure 2: Sequence alignment of Orf GIF and members of the Poxviral Immune Evasion (PIE) family.
From: Structural basis of GM-CSF and IL-2 sequestration by the viral decoy receptor GIF
Structural alignment performed by Expresso76 and visualized by ESPript77. Used sequences include Orf virus (ORF) CKBP, vaccinia virus (VV) A41, ectromelia virus (EV) vCCI (EVM1), cowpox virus (CPV) VCCI, rabbitpox virus (RPV) vCCI, cowpox virus (CPV) CPXV203 and ectromelia virus (EV) SECRET domain of CrmD. Conserved negatively and positively charged residues are shown in red and blue respectively, conserved polar residues are shown in light blue and conserved hydrophobic residues are shown in orange. Fully conserved residues are coloured black, with a yellow outline for cysteine. Conserved disulfide bonds are shown as connecting dashed lines. Secondary structure elements in GIF are annotated above the corresponding sequences. Residues of GIF involved in interactions with GM-CSF are shown as circles (red: interaction with helix αA, blue: interaction with helix αD of GM-CSF), whereas N-linked glycosylation sites in GIF are marked with a green star.
