Figure 4: H2O2-induced potentiation of sensory behavior and ASH neuron function requires peroxiredoxin-p38/PMK-1 signaling.
From: Promotion of behavior and neuronal function by reactive oxygen species in C. elegans
(a) H2O2-induced behavioral potentiation requires NSY-1/ASK1, SEK-1/MKK, and PRDX-2/peroxiredoxin. In nsy-1(ok593), sek-1(km4) and prdx-2(gk169) mutant worms, H2O2 treatment failed to promote osmotic avoidance behavior. RNAi of nsy-1 gene in ASH neurons of wild-type worms recapitulated the phenotype. This was done by expressing nsy-1 RNAi as a transgene in ASH neurons under the sra-6 promoter. The sek-1 and prdx-2 mutant phenotype was rescued by a transgene expressing sek-1 and prdx-2 cDNA in ASH neurons driven by the sra-6 promoter, respectively. n≥20; **P<0.005, ***P<0.0005 (ANOVA); Error bars: s.e.m. (b,c) H2O2-induced potentiation of ASH sensory response requires NSY-1. In nsy-1 mutant worms, H2O2 treatment can no longer promote ASH calcium response to glycerol stimulus (b). Shades along the traces in (b) represent error bars (s.e.m.). Bar graph in (c) summarizes the data in (b). n≥8; Error bars: s.e.m. (d,e) H2O2-induced potentiation of ASH sensory response requires SEK-1. In sek-1 mutant worms, H2O2 treatment can no longer promote ASH calcium response to glycerol stimulus (d). Shades along the traces in (d) represent error bars (s.e.m.). Bar graph in (e) summarizes the data in (d). n≥9; Error bars: s.e.m. (f,g) H2O2-induced potentiation of ASH sensory response requires PRDX-2. In prdx-2 mutant worms, H2O2 treatment can no longer promote ASH calcium response to glycerol stimulus (f). Shades along the traces in (f) represent error bars (s.e.m.). Bar graph in (g) summarizes the data in (f). n≥8; Error bars: s.e.m.
