Figure 1: Identification of Arabidopsis SWEET proteins as candidate GA transporters.
From: AtSWEET13 and AtSWEET14 regulate gibberellin-mediated physiological processes
(a) A phylogenetic tree of 17 Arabidopsis SWEET proteins was generated by ClustalX using the neighbour-joining method and displayed using Njplot. The scale bar indicates a branch length corresponding to 0.5 substitutions per site. (b) The effects of six closely related AtSWEET proteins on the interaction between GID1a and GAI determined by Y2H assays using HIS3 as a selection marker. AtSWEET9, AtSWEET10, AtSWEET11, AtSWEET12, AtSWEET13 or AtSWEET14 was expressed in yeast containing the Y2H system with BD-GID1a and AD-GAI, and grown on selection media (SD; -Leu, -Trp, -Ura, -His) containing GA1 (0.1 μM), GA3 (0.1 μM) or GA4 (1 nM) for 7 days. Yeast transformed with an empty vector was used as a control. 1 × 104 cells were inoculated on the media. (c) The effects of six closely related AtSWEET proteins on the interaction between GID1a and GAI were determined by Y2H assays based on the expression of the lacZ marker. Units of β-galactosidase (β-gal) activity represent 1,000 × OD420/min/OD600 cells. Values are means±s.d. of three biological replicates.
