Figure 2: Architecture of the PRC (30S·ABCE1·AMP-PNP) mapped by XL-MS. | Nature Communications

Figure 2: Architecture of the PRC (30S·ABCE1·AMP-PNP) mapped by XL-MS.

From: Structure of the ribosome post-recycling complex probed by chemical cross-linking and mass spectrometry

Figure 2

(a) The orientation of ABCE1 in the PRC based on the identified inter cross-links with the archaeal ribosomal proteins S24e (pink, b) and S12 (dark magenta, c), depicted in blue and red lines. Blue lines indicate cross-links with a length<30 Å and red lines cross-links>30 Å. Identified inter cross-links were certified using an in silico model of the S. solfataricus 30S constructed by aligning the homology models of the archaeal ribosomal proteins to the small ribosomal subunit from S. cerevisiae (pdb: 3U5G/F, r-proteins: cyan, rRNA: grey) and positioning ABCE1 according to the cryo-EM map of the rescue/pre-recycling complex (pdb: 3J16). (d) The major contact area of ABCE1 towards the 30S primarily locates in the helix–loop–helix region (HLH).

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