Figure 6: Identification of new functional sites controlling insulin secretion.

(a) Phosphorylation profile of the potential regulatory phosphosites. (b) Experimental strategy applied to profile the phosphoproteome in a glucose time course experiment. Lower part: PCA of Min6 beta cells treated for the indicated time points with glucose. (c) Heat map of the Log2 LFQ intensities of the potential regulatory phosphosites significantly modulated in the time course experiment. (d) For the four known regulatory sites, the median fold-changes and s.e.m. compared with starvation control cells are plotted. (e) Heat map of the Log2 LFQ intensities of 10 novel regulatory phosphoproteins significantly modulated in pre-diabetic and diabetic NOD islets.