Figure 6: CaMKv knockdown in hippocampal CA1 neurons leads to hyperactivity and reduced MWM performance.
From: The pseudokinase CaMKv is required for the activity-dependent maintenance of dendritic spines
(a–c) CaMKv knockdown mice exhibited hyperlocomotion in the open-field test, significantly increased total running distance and central/total duration (Con: n=9 mice, shCaMKv: n=12 mice, **P<0.01, Student’s t-test). (d–g) CaMKv knockdown reduced MWM performance. (d) Mice were trained in the MWM and tested in a probe trial on day 8. The acquisition over 7 days (four trials per day) was similar between groups. (e) Representative swimming paths of a control mouse and a CaMKv knockdown mouse are shown. (f) In the probe trial, control mice spent significantly more time in the target quadrant (T) than any other quadrant (T versus L, T versus O or T versus R, ***P<0.001, one-way analysis of variance (ANOVA), Tukey’s multiple comparison test). The CaMKv knockdown mice spent comparable time in T as the other quadrants at the level of chance (T versus L, T versus O or T versus R, P>0.05, one-way ANOVA, Tukey’s multiple comparison test). Time spent in T was significantly lower in the CaMKv knockdown mice than the controls (Con: n=14 mice, shCaMKv: n=18 mice, ***P<0.001, two-way ANOVA, genotype × platform: F(3,90)=5.49, P<0.01, genotype: F(1,30)=−0.4835, platform: F(3,90)=16.05, P<0.0001, Bonferroni’s multiple comparison test). (g) The cumulative distance of the swimming path to the platform was greater in CaMKv knockdown mice than the controls (Con: n=14 mice, shCaMKv: n=18 mice, ***P<0.001, two-way ANOVA, genotype × platform: F(3,120)=6.48, P<0.001, genotype: F(1,120)=−2.16, platform: F(3,120)=17.92, P<0.0001, Bonferroni’s multiple comparison test).
