Figure 3: Progressive lipid ovoid deposition was visualized by long-term serial in vivo SRS imaging. | Nature Communications

Figure 3: Progressive lipid ovoid deposition was visualized by long-term serial in vivo SRS imaging.

From: Monitoring peripheral nerve degeneration in ALS by label-free stimulated Raman scattering imaging

Figure 3

(a) Experimental design of long-term serial in vivo SRS imaging. (b) Stage alignment for in vivo SRS imaging of sciatic nerves. (c) Incision size of a representative imaged mouse. Scale bar, 1 cm. (d–f) SOD1G93A versus WT long-term serial in vivo SRS images at different ages during disease progression. Scale bar, 50 μm. (g) Quantification of lipid ovoids for long-term serial in vivo SRS imaging throughout disease progression. *P<0.05, ***P<0.001. N=5 for each strain at each time point. For 5-week-old mice, P=0.043. For 8-week-old mice, P=0.00012. For 11-week-old mice, P=0.00031. Data are presented as mean±s.e.m., and error bars show s.e.m.

Back to article page