Figure 8: SRS imaging identified lipid ovoid deposition in human ALS patient samples.
From: Monitoring peripheral nerve degeneration in ALS by label-free stimulated Raman scattering imaging
(a) Experimental design of SRS imaging for human ALS patient samples. (b) Lipid ovoid quantification of ventral (motor fiber enriched) and dorsal (sensory fiber enriched) root samples from either human ALS patients or controls. *P<0.05. N=4 for each group. Data are presented as mean±s.e.m., and error bars show s.e.m. (c) SRS imaging of ventral root nerve fibers from human ALS patients versus controls. Scale bar, 50 μm. (d) SRS imaging of dorsal root nerve fibers from human ALS patients versus controls. Scale bar, 50 μm. (e) Timeline of ALS associated pathological changes and diagnostic methods. EMG, electromyography; IHC, immunohistochemistry; MN, motor neuron; NMJ, neural muscular junction; SRS, stimulated Raman scattering; TEM, transmission electron microscope.
