Figure 6: K-mer comparison
From: TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol
K-mers were calculated with jellyfish. The x axis shows the k-mer bin size, the y axis shows the frequency of the corresponding k-mer bin in per cent. The NA sample and TruePrime show a clear separation of signal k-mer from error noise at low bin sizes, the commercial RP-MDA kit and MALBAC display no such separation. The estimated genome sizes E were calculated on the basis of the k-mer distribution as 
, where B is the bin size, F is the corresponding frequency and P is the peak depth estimated from the k-mer distribution. Only k-mer bins with size >4 were included, as lower bin sizes tend to be random noise originating from the library construction and sequencing process. Single-copy region contains the amount of k-mers covering the single copy peak from bin size 4 to bin size 50.
