Figure 7: Reproducibility in TruePrime-amplified single cells.
From: TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol
(a) Circos plot of genome coverage from NA material (grey) and four HEK293 cells amplified with TruePrime (blue) (input: exactly five million randomly selected read pairs). The coverage pattern appears very similar in the 4 replicates. It is noteworthy that no major part of any chromosome is missing in the amplified DNA. (b) Pearson’s product–moment cross-correlation of binned read depth (bin size=100 kb) for all four replicates. (c) Sliding window coverage comparison of chromosome 4 between NA and the four replicates. Again, the coverage pattern is highly similar between the replicates. For a close-up view, see Supplementary Fig. 8.
