Figure 10: X–Y chromosome behaviour.
From: C14ORF39/SIX6OS1 is a constituent of the synaptonemal complex and is essential for mouse fertility
(a) Double immunolabelling of SYCP3 (red) and SYCP1 (green) in wild-type pachytene, and Six6os1−/− and Syce3−/− pachytene-like arrested spermatocytes. Yellow letters indicate aligned/unaligned sex chromosomes in mutant spermatocytes. (b) Co-labelling of SYCP3 (red) and γ-H2AX (green) in Six6os1−/− pachytene-like arrested spermatocytes, showing moderate staining in XY chromosomes. (c) Double immunolabelling of SYCP3 (red) and 53BP1 (green). 53BP1 signal is not observed in the XY chromosomes of mutant spermatocytes, in contrast to the strong labelling of the sex body in wild type. Scale bar, 10 μm.
