Figure 9: SIX6OS1 is essential for the processing of intermediate recombination nodules.
From: C14ORF39/SIX6OS1 is a constituent of the synaptonemal complex and is essential for mouse fertility
(a) Double immunolabelling of SYCP3 (red) with MLH1 (green). MLH1 foci are absent at the AEs/LEs of Six6os1−/− meiocytes whereas at least one focus is present along each autosomal SC in wild-type pachytene meiocytes. (b) Immunostaining of SYCP3 (red) and ACA (green) in wild type and Six6os1−/− spermatocytes. Okadaic acid-induced metaphase I plates of wild-type spermatocytes give rise to 20 bivalents each, with two opposed centromere signals (ACA) and positive staining for SYCP3, whereas Six6os1−/− spermatocytes lead to 40 free univalents, each with an ACA signal and SYCP3 labelling the centromeric and interchromatid domain. Scale bar, 10 μm.
