Figure 1: AAA+ proteases at the mitochondrial inner membrane. | Nature Communications

Figure 1: AAA+ proteases at the mitochondrial inner membrane.

From: Engineered AAA+ proteases reveal principles of proteolysis at the mitochondrial inner membrane

Figure 1

(a) YME1L and AFG3L2/Paraplegin are two homologous human AAA+ proteases anchored to the inner membrane of mitochondria where they degrade protein substrates in both soluble compartments and the inner membrane (OM=outer membrane; IMS=intermembrane space; IM=inner membrane; M=matrix). (b) Each YME1L subunit contains an N-terminal domain (blue), transmembrane span (yellow), AAA+ ATPase domain (green) and M41 zinc metalloprotease domain (pink). The YME1L-AP construct contains the AAA+ ATPase domain and M41 protease domain (residues 317–773). In hexYME1L, the N-terminal domain and transmembrane span are replaced by the 30-residue cc-hex coiled coil sequence (orange). The E439Q substitution blocks ATP hydrolysis activity.

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