Figure 5: The C termini of TIM17A and TIM17B can promote degradation.
From: Engineered AAA+ proteases reveal principles of proteolysis at the mitochondrial inner membrane
(a) Cartoon showing organization of the TIM23 translocase complex. Known subunits of the complex include TIM23 (green), TIM50 (blue), TIM44 (red) and TIM17 (orange). The C-terminal tail of TIM17 is predicted to project into the intermembrane space. (b) Protein sequence alignment of human TIM17A and TIM17B. Identical aligned residues are coloured yellow and conservative substitutions are coloured grey. (c) SDS–PAGE showing degradation of I27CD-17A and I27CD-17B (20 μM) by hexYME1L (1 μM) in the presence or absence of ATP. (d) Loss of I27-Tim17 variants over time from degradation experiments in c. **P≤0.01 calculated from an unpaired Student’s t-test (two-tailed). All data shown are from independent experiments and error bars indicate±s.e.m. (n=3).
