Figure 6: YME1L discriminates between degrons by sequence.
From: Engineered AAA+ proteases reveal principles of proteolysis at the mitochondrial inner membrane
(a) Sequences of each degron fused to cp7-SFGFP and tested for ATP-dependent degradation by hexYME1L. (b) Michaelis–Menten plots showing degradation of cp7-SFGFP variants bearing C-terminal fusions of degrons previously shown to enhance degradation by AAA+ proteases (β20 (green) and sul20C (blue), Lon protease; ssrA (red), ClpXP protease; β10 (orange); extssrA (grey)). Lines are nonlinear least-squares fits to the Michealis–Menten equation. (c) Michaelis–Menten plots of cp7-SFGFP proteins bearing C-terminal fusions of multiple truncated variants of the β20 degron (β10 (orange); β5, (pink); βF (magenta); βF2 (cyan); β5F (yellow)). Lines are nonlinear least-squares fits to the Michealis–Menten equation. (d) Maximal degradation rates (black columns) and KM values (white columns) for each degron sequence fused to the C-terminus of cp7-SFGFP. All degradation reactions contained hexYME1L (1 μM). All data shown are from independent experiments and error bars indicate±s.e.m. (n=3).
