Table 1 Degradation of GFP proteins bearing different degron sequences.
From: Engineered AAA+ proteases reveal principles of proteolysis at the mitochondrial inner membrane
Degron | Number of residues | Sequence | kdeg (min−1enz6−1) | KM (μM) |
|---|---|---|---|---|
β20 | 20 | QLRSLNGEWRFAWFPAPEAV | 0.110±0.003 | 7.1±0.6 |
ssrA | 11 | AANDENYALAA | 0.031±0.004 | 12.0±4.0 |
sul20C | 20 | ASSHATRQLSGLKIHSNLYH | 0.036±0.005 | 15.2±5.0 |
β10 | 10 | FAWFPAPEAV | 0.105±0.015 | 12.4±1.9 |
extssrA | 19 | QLRSLNGEAANDENYALAA | 0.106±0.007 | 20.4±3.1 |
β5 | 5 | APEAV | ND | ND |
βF | 5 | FAWFP | 0.040±0.003 | 18.9±3.0 |
βF2 | 10 | FAWFPFAWFP | 0.044±0.003 | 1.3±0.4 |
β5F | 10 | APEAVFAWFP | 0.035±0.002 | 6.1±0.6 |
