Figure 3: Interaction between VPS35 and TBC1d5.
From: Structural and mechanistic insights into regulation of the retromer coat by TBC1d5
(a) SAXS scattering curves. The VPS35/VPS29 binary and VPS35/VPS29/TBC1d5TBC ternary scattering curves are shown as black points. The CORAL rigid-body model fits are shown as red (Binary) and blue (Ternary) lines. Inset: the Guinier plot for each data set is shown with the linear fit coloured as in the figure. The Ternary curve is offset for clarity. (b) P(r) curves. The Binary (blue) and Ternary (red) pair–distance distribution functions as determined by BayesApp. (c) SAXS ab initio molecular shapes. Overlaid Dammif bead models, Binary (red) and Ternary (blue) are shown in two different views. (d) VPS35 WT, but not Δ6, co-immunoprecipitated with TBC1d5. Hela cells were transfected with yellow fluorescent protein (YFP) or various YFP–VPS35 and immunoprecipitate with anti-VPS35 antibodies. TBC1d5, VPS26, VPS29 and green fluorescent protein (GFP) were immunoblotted. See also Supplementary Fig. 8.
