Figure 1: TGFβ induces nuclear accumulation of TβRI intracellular domain (TβRI-ICD). | Nature Communications

Figure 1: TGFβ induces nuclear accumulation of TβRI intracellular domain (TβRI-ICD).

From: TRAF6 ubiquitinates TGFβ type I receptor to promote its cleavage and nuclear translocation in cancer

Figure 1

(a, b) Knockdown of TβRI by siRNA was performed to demonstrate the specificity of the V22 and H100 antibodies, which recognize the intracellular and extracellular domain of TβRI, respectively, in immunofluorescence (a; DAPI was used to visualize cell nuclei, scale bar 20 μm). (b) Cell lysates of PC-3U cells treated or not with TGFβ, were fractionated into cytoplasmic and nuclear proteins and subjected to SDS–gel electrophoresis, followed by immunoblotting using V22 and H100 antibodies. β-tubulin and lamin A served as controls for the cytoplasmic and nuclear fractions, respectively. Molecular weight markers are indicated. (c) Representative confocal microscopy pictures of C-terminally pEGFP-N3-tagged wt TβRI (GFP- TβRI) and pEGFP-N3-vector (GFP) expressed in PC-3U cells treated with TGFβ for 6 h are shown (left panel) and quantified in right panel (mean±s.d., n=3–5 independent experiments where N=350 cells where counted in each group, *P<0.0002, ANOVA). Cell nuclei were stained with DAPI. Scale bar 20 μm.

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