Figure 2: STING is essential for restriction of HSV-1 in microglia in vitro and for antiviral control in neurons in vivo.
From: Sensing of HSV-1 by the cGAS–STING pathway in microglia orchestrates antiviral defence in the CNS
(a–c) Astrocytes, neurons and microglia from WT and Stinggt/gt mice were cultured in vitro and infected with HSV-1 (MOI 1). Supernatants were collected 48 h later and virus was quantified by plaque assay. Data are presented as means ± s.e.m. *0.01<P<0.05; NS, not significant, n=5–8 per group. (d–g) Tissue sections from the brain stem of six WT and 6 Stinggt/gt mice isolated 6 days after infection with HSV-1 (1 × 106 PFU per eye) were stained with an antibody against HSV-1 and antibodies against cell-type-specific markers: (d) S100, a nuclear astrocyte marker; (e) GFAP, a fibrillary astrocyte marker; (f) NeuN, neurons; and (g) Iba1, microglia. Scale bar, 20 μm. Cells marked by arrow-heads are magnified in the images to the left and right of the large images in d,f and g. The magnified images in g show staining for HSV-1 and DAPI without the cell-type-specific marker (Iba1).
