Figure 5: Inhibition of cell death in LUBAC-deficient thymocytes. | Nature Communications

Figure 5: Inhibition of cell death in LUBAC-deficient thymocytes.

From: Linear ubiquitin chain assembly complex coordinates late thymic T-cell differentiation and regulatory T-cell homeostasis

Figure 5

(a) Cell death determined by PI uptake in thymocytes from control, HoipΔCd4, HoilΔCd4 and Sharpincpdm mice cultured for 24 h with combinations of agonists (T: 100 ng ml−1 TNF; S: 500 nM SMAC mimetic; Q:10 μM QVD-OPh;N: 10 μM necrostatin). Percentages (b) and absolute numbers (c) of CD4+FOXP3+ cells in the thymus of WT (n=18), Sharpincpdm (n=11), Mlkl−/− (n=4), Mlkl−/−Casp8−/− (n=4), SharpincpdmCasp8−/−Mlkl−/− (n=4), Rip3k−/−Casp8+/− (n=4), SharpincpdmRip3k−/−Casp8+/− (n=4), Tnf−/− (n=4) and SharpincpdmTnf−/− (n=5) mice. Shpncpdm refers to Sharpincpdm mice. (d) Surface expression of CD4 and CD8 on whole thymocytes from control, Casp8ΔCd4Mlkl−/−, HoilΔCd4 and HoipΔCd4Casp8Δcd4Mlkl−/−mice. Data are representative of two independent experiments with one mouse per genotype (a,d). For b and c, each symbol represents an individual mouse, with 4–14 mice per group; small horizontal lines indicate mean±s.d.; *P<0.05, **P<0.01, ***P<0.005 and ****P<0.001, respectively. One-way analsyis of variance with a Tukey’s post-hoc test for multiple comparisons was used for statistical analysis.

Back to article page