Figure 5: CELF1 expression and function are conserved in multiple cellular models of EMT.
From: CELF1 is a central node in post-transcriptional regulatory programmes underlying EMT
(a) Immunoblot analysis of indicated epithelial and mesenchymal cell markers and CELF1 in untreated or TGF-β-treated MCF10A, MCF10AT1, MCF10CA1a and HMLE cells, and in untreated or EGF-treated MDA-MB-468 and MDA-MB-231 cells. HSP90 serves as a loading control. (b) Quantification of cellular migration and invasion in transwell assays in untreated or TGF-β-treated MCF10A, MCF10AT1, MCF10CA1a and HMLE cells, and in untreated or EGF-treated MDA-MB-468 and MDA-MB-231 cells. (c,d) As in a and b, but in response to stable Renilla Luciferase or CELF1 overexpression in the indicated cell lines, respectively. In c, HSP90 serves as a loading control. (e,f) As in a and b, but in response to stable knockdown of β-galactosidase (shGLB1) or two distinct CELF1 shRNAs (shCELF1A, shCELF1B) in the indicated cell lines, respectively. In e, HSP90 serves as a loading control. All panels are representative of a minimum of three individual experimental replicates. For immunoblots depicted, samples were derived from the same experiment and gels were processed in parallel. Error bars depict s.d. of the mean. *P≤0.05 (Student’s t-test). See also Supplementary Fig. 4. Full scans of blots are shown in Supplementary Fig. 11.
