Figure 3: Lungfish Mlp expression on regenerating fin.

(a) Sequence alignment of MLPs as performed using a similarity matrix BLOSUM62 to compare sequences from human (Homo sapiens); chicken (Gallus gallus); frog (Xenopus laevis); axolotl (Ambystoma mexicanum); lungfishes Lepidosiren paradoxa and Protopterus annectens; and coelacanth (Latimeria chalumnae). Black bars highlight the three MLP conserved domains: myristoylated N terminus domain (MD), MARCKS homology domain (MHD) and effector domain (ED). Serines in red are potential target for phosphorylation by PKC. (b) Phylogenetic tree of MARCKS (blue) and MLP (red) protein orthologs was inferred using the Neighbour-Joining method. Bootstrap percentage values are shown next to the branches and the evolutionary distances were computed using the p-distance method. (c) Relative expression for the lungfish Mlp, using two sets of primers (Mlp 5′ in black, Mlp 3′ in grey), was obtained for FB, 1, 7 and 14 dpa, normalized to expression level in NRF to a relative value of 1.0 (n=1 biological sample, three technical replicates); mean±s.d.