Figure 4: Lungfish LSG expression in the FB and other adult tissues.

(a) Volcano plot showing differentially expressed LSGs between NRF tissue and FB at 3 wpa. Each dot represents a gene and coloured dots denote four LSGs selected for further analysis (c19141, yellow; c19958, orange; c28232, green; c29579, blue). Black and grey dots represent genes below or within the P value cut-off, respectively, which is denoted by a dashed line Student’s t-test P value (<0.05). Fold change is displayed in a log₂ scale and P value in −log₁₀. (b) RNA-seq read coverage of the four candidate LSG transcripts shown in black, the predicted open reading frames (ORF) is shown in colour for each LSG, according to colour scheme in a. (c) Relative expression for the four, colour coded, candidate LSGs in various tissues is shown, normalized to expression level in NRF to a relative value of 1.0 (dashed line) (n=1 biological sample, three technical replicates); mean±s.d.; PV, P value; FC, fold change.