Figure 10: Reduction of pre-labelled PP hepatocytes after injury induced by BDL.
From: Mfsd2a+ hepatocytes repopulate the liver during injury and regeneration
(a) Schematic figure showing strategies for PP hepatocytes labelling and injury model induced by BDL. (b) Whole-mount bright view of sham and BDL livers. Scale bars, 2 mm. (c) Sirius red staining of liver sections. Scale bars, 200 μm. (d) Whole-mount fluorescence view of sham and BDL livers. Inserts are bright-field images of the same liver. Scale bars, 500 μm. (e) Immunostaining for RFP, CK19 and HNF4a on sham or BDL liver sections. Boxed regions are magnified in lower panels. Insertions indicate the quantification of the percentage of RFP+ hepatocytes in sham or BDL livers. n=4. Error bars are s.e.m. of the mean. Scale bars, 500 μm. Each image is a representative of four individual samples.
