Figure 6: PP hepatocytes expand and replace almost all hepatocytes in the liver lobule after injury.
From: Mfsd2a+ hepatocytes repopulate the liver during injury and regeneration
(a) Schematic figure showing experimental strategy for tamoxifen induction and CCl4 treatment. (b) Sirius red staining images showing robust fibrotic responses in CCl4-treated group, compared with oil-treated group (control). Scale bars, 1 mm. (c) Whole-mount fluorescence view of Mfsd2a-CreER;Rosa26-RFP livers from control (left) and CCl4 (right)-treated groups. n=4. Error bars are s.e.m. of the mean. Scale bars, 1 mm. (d) Immunostaining for RFP, CK19 and HNF4a on Mfsd2a-CreER;Rosa26-RFP liver sections of control and CCl4-treated mice. Scale bars, 100 μm. (e) Sequential whole-mount fluorescence view of the same liver from individual mouse before injury and at week 4 after chronic injury. Scale bars, 1 mm. (f) Schematic figure showing expansion of PP hepatocytes after injury. CV, central vein; PV, portal vein. Each image is a representative of four individual samples.
