Table 2 Data from the 2D AUC distributions for both nanoclusters and PDT-capped nanoparticles.

From: Determination of nanoparticle size distribution together with density or molecular weight by 2D analytical ultracentrifugation

Clusters

s (S)

D (cm2 s−1)

d p (nm)

ρP (g cm−3)

M by AUC (Da)

M by ESI-MS

MW error (%)

Au25(SR)18− TOA+

6.8±0.1

2.8×10−6

2.41±0.02

1.95

8,000±90

7,862

1.75

Au38(SR)24

9.6±0.1

2.7×10−6

2.58±0.01

2.43

11,030±50

10,778

2.33

Au144(SR)60

27.1±0.4

2.5×10−6

2.83±0.01

4.51

35,260±180

36,598

3.65

PDT-NPs

s (S)

D (cm2 s−1)

d p (nm)

ρP (g cm−3)

M by AUC (Da)

d core (nm)

d core from TEM (nm)

Peak A

985±13

1.2×10−6

11.54±0.15

4.65

2,256,790±25,300

6.82±0.15

6.7±0.07

Peak B

1022±21

1.4×10−6

10.37±0.22

5.80

2,037,690±34,820

6.68±0.22

 
  1. Experimental data for Figure 3. (top) Three Au nanocluster standards (R=−CH2CH2Ph). Presented are the measured weight-averaged sedimentation coefficients (toluene, 20 °C), diffusion coefficients, hydrodynamic diameter, density, molecular weights by both AUC and ESI-MS, and average molecular weight percent errors from molecular formula as determined by ESI-MS. The standard deviation in s was taken as the full width at half maximum, which was then propagated through the calculations. (bottom) For both peaks of the PDT-NPs, the 2D distributions were transformed into density, molecular weight, and particle and core diameter. Also included is the average core diameter from TEM, in order to compare with the core diameters measured for both peaks by AUC.
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