Figure 6: HCys-induced sulfcatalase formation is likely in various pathological disorders.

(a) Relative specific activity of immunocaptured catalase (SpCAT) in various human colorectal cancer cells. The HT-29 Glc−/+ cell line was chosen as a control as it maintains metabolic characteristics of normal colonocytes. (b) Comparison of SpCAT in human mammary epithelial cells (HMEC-control) and in various HBC cells. (c) SpCAT measured in diverse cellular models of neurodegenerative disorders: Hek 293T cells transfected with an empty plasmid (pcDNA-control), Htt-N171-82Q (Htt) or Alpha-synuclein-A53T (α-Syn) and M17 human neuroblastoma cells treated or not (control) with 100 nM rotenone. (d) SpCAT in crypt and surface epithelial cells isolated from a rodent model of DSS-induced colitis (DSS) in comparison with untreated mice (control). (e–g) Dependency of H2O2 consumption in various cell lysates on the concentration of NaSH. Cell lysates were preincubated with NaSH on ice for 5–10 min before monitoring H2O2 disappearance at 240 nm. (h,i) Dependency of SpCAT on the concentration of NaSH in various human colorectal cancer cells (h) and in a cellular model of Parkinson’s disease (i). Data are represented as means±s.d. (colorectal cancer cells and neurodegenerative diseases, n=2; breast cancer cells and colitis, n=3). Each experiment was performed in duplicate (SpCAT) or triplicate (H2O2 consumption).