Figure 5: Phylogenetic trees presenting relationships between FLC and AOP2 gene protein products.
From: The pangenome of an agronomically important crop plant Brassica oleracea
(a) There are six FLC genes identified in the pangenome. BrFLC1, 2, 3, 5 and BoFLC1, 3, 5 are protein products of B. rapa and B. oleracea FLC genes, respectively, previously identified by Schranz et al.27. Bo3g005470, Bo3g024250, Bo9g173370 and Bo9g173400 are protein products of genes identified on the TO1000 portion, and BOLEPAN_00002684 and BOLEPAN_00005424 on the newly assembled portion of the pangenome. (b) There are four AOP2 genes identified in the pangenome. AOP1V_ARATH, AOP2V_ARATH and AOP3L_ARATH are protein products of AOP1, 2, 3 genes identified in A. thaliana. BolAOP2 is a protein product of the only full-length AOP2 gene identified in B. oleracea var capitata genome1. Proteins with IDs beginning with Bra are products of AOP genes identified in B. rapa and proteins with IDs beginning with Bo are products of AOP genes identified in the B. oleracea pangenome in this study. Genes Bo9g006220 and Bo9g006240 co-localize with quantitative trait locus (QTL) controlling amount of several glucosinolates70. Genes displaying PAV are highlighted in blue rectangles. Scale bars indicate number of amino-acid substitutions per site.
