Figure 4: SLRB-iHPs generate B cells in vivo.
From: Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors
V(D)J recombination events and transgene integration were detected in SLRB-iHP-derived B cells (iHP-B cells) at single-cell level. iHP-B cells are from 16 wpt spleens. Numbers at top of the panel are cell ID of individual single cells. Con (B6) denotes control (Con) cells from the SP of C57BL/6 (B6) mice. MEF (14 dpi) are cells from SLRB infected tdTomato+ MEF at 14 dpi. Sp (B6) and Sp (tdTomato+) stand for total spleen cells. VH7183-DJH and DHQ52-JH are used to reveal V to DJ and D to J recombination events at heavy chain, respectively. Vk−Jk denotes V to J recombination at Kappa chain. WT (Wild type) and MT (tdTomato mutant) show the genotype of the cells. SLR-T and B-T stand for transgene integration of SLR (Polycistronic) and Bmi1. Polycistronic SLR was used for the generation of iHP-B cells and 14 dpi MEF. These are representative of two independent experiments.
