Figure 1: H4K5 and K12 acetylation are detected in centromeres.
From: Acetylation of histone H4 lysine 5 and 12 is required for CENP-A deposition into centromeres
(a) High-resolution profile of ChIP-seq analysis with anti-CENP-A, anti-H4K20me1 or various antibodies against H4 modifications including K5ac, K8ac, K12ac, K16ac and K20ac around centromere region of chromosome Z (42.55–42.725 Mb). (b) ChIP-seq analysis with anti-CENP-A or anti-H4K5ac antibodies on chromosome Z in DT40 cells. Sequence reads were mapped for CENP-A at 100 kb window and for H4K5ac at 100 kb and 10 kb windows. At 10 kb windows a peak for H4K5ac at centromere position are clearer. (c) ChIP-seq analysis with anti-CENP-A or anti-H4K12ac antibodies on chromosome Z in DT40 cells. Sequence reads were mapped for CENP-A at 100 kb window and for H4K12ac at 100 kb and 10 kb windows. At 10 kb windows a peak for H4K12ac at centromere position are clearer. (d) Immunofluorescence analysis with Cy3-labelled anti-H4K12ac antibody (red) in HeLa cells expressing CENP-A-GFP (green). Co-localization of H4K12ac with CENP-A was observed (merge). Typical centromere signals are shown in yellow arrows. Bar, 10 μm.
