Figure 5: PolII S2P binding, CRTC binding and histone acetylation are regulated by GCN5, Tip60 and CRTC. | Nature Communications

Figure 5: PolII S2P binding, CRTC binding and histone acetylation are regulated by GCN5, Tip60 and CRTC.

From: Shifting transcriptional machinery is required for long-term memory maintenance and modification in Drosophila mushroom bodies

Figure 5

(a,b) Histone acetylation and CRTC binding after spaced training are abundant in comparison with those after massed training at Bx and Smr gene loci. The purified MB nuclei expressing FLAG-KASH prepared from naive control flies or flies 1 day after spaced training or massed training were subjected to ChIP analysis using anti-H3K9Ac, anti-H4K16 or anti-CRTC antibodies. The ChIP DNA was analysed at Bx (a) or Smr (b). The naive control flies were used to calculate relative enrichment (n=3 for all data.). (c,d) PolII S2P binding, CRTC binding and histone acetylation are reduced by knockdown of GCN5, Tip60 and CRTC. Flies expressing FLAG-KASH in the MBs and carrying MBsw were crossed with the UAS-RNAi lines, as indicated at the bottom of each panel. The MB nuclei from naive control flies and flies 1 day after spaced training were collected as in a and subjected to PolII S2P ChIP assay. The ChIP DNA was analysed at Bx (c) or Smr (d). The naive control flies were used to calculate relative enrichment (n=3 for all data). *P<0.05 determined by Student’s t-test.

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