Figure 3: Pooled data showing the effect of activation of different opsins on EGABA.
From: Cl-out is a novel cooperative optogenetic tool for extruding chloride from neurons
The data show the shift in IPSP peak (muscimol puff, see Fig. 2) following a brief, opsin activation (5 s continuous activation unless otherwise indicated), measured in perforated patch recordings of dissociated cultured neurons. In some cases, cells were preloaded with chloride by blocking KCC2 (using VU0463271, ‘VU’) and NKCC1 (bumetanide, ‘Bume’). Numbers of cells recorded in each set are given in brackets.
