Figure 5: Reversal of excitable network state by Cl-out1 activation.
From: Cl-out is a novel cooperative optogenetic tool for extruding chloride from neurons
(a) Field recordings in six brain slices prepared from six young adult mice transfected with Cl-out1 (injected AAV viral vector on the day of birth). Epileptiform activity was induced by 30 min prior incubation in VU0463271. Red dots indicate times of electrical stimulation, and expanded sample events are shown below (b). The VU-induced excitability was reversed consistently by a period of Cl-out activation, performed intermittently (25 s light on, 5 s light off), with the electrical stimulation occurring in the dark period of the cycle. There were highly significant reductions both of spontaneous events (c) and the multiunit bursts in response to electrical stimuli, assessed by the power in the 300–600 Hz frequency band (d).
