Figure 5: Net4 induces neurite outgrowth in a laminin-dependent manner.
From: Structural decoding of netrin-4 reveals a regulatory function towards mature basement membranes
(a) Diagram depicting the olfactory bulb (OB) explants assay procedure. (b) Representative images of untreated (ctrl), laminin γ1 (γ1LN-LEa1-4)-, Net4 (Net4-ΔC)-, Net4 in combination with laminin γ1 (Net4-ΔC+γ1LN-LEa1-4)-, and Net4 laminin-binding mutant (Net4-ΔCE195A,R199A)-treated OB explants. (c) Statistical analysis of OB explants (mean±s.e.m.; n=19; ****P<0.0001, ns, not significant). Error bars, s.e.m. (n=19 independent cell cultures). P values, two sided t-test. Scale bar, 200 μm (b).
