Figure 2: Robo4 inhibits OIR revascularization independently of its cytoplasmic domain.
From: The Robo4 cytoplasmic domain is dispensable for vascular permeability and neovascularization
(a) IsoB4-stained whole-mount retinal vasculature of P17 mice with the indicated genotypes after OIR. SRobo4 was injected intraperitoneally daily from P12 to P16. Scale bar, 500 μm. (b) Higher magnification images of boxed areas in a showing vessels sprouting from veins. Scale bar, 100 μm. (c) Quantifications of avascular area in the whole retinas shown in a. (d) Quantifications of vessel coverage in the red stippled area in b. Each dot represents a retina. N=12–34 retinas (6–17 mice) per group. Error bars: s.e.m. ***P<0.001, Mann–Whitney U test. (e) IsoB4-stained neovessel tufts of P17 mice with the indicated genotypes after OIR. To preferentially stain neovascular tufts (arrowheads), retinas were subjected to mild detergent permeabilization and short IsoB4 incubation. Scale bar, 500 μm. (f) Quantifications of tuft area in the retinas shown in e. N=8–16 retinas (4–8 mice) per group. Error bars: s.e.m. No significant differences in tuft area were observed using Mann–Whitney U test.
