Figure 1: Maintained Ad5 infectivity versus time in PBS and sucrose.
From: Additives for vaccine storage to improve thermal stability of adenoviruses from hours to months
(a) Fraction of cells infected versus storage time (days) of Ad5 in PBS at RT (blue) and at 37 °C (red). The curves are fits to the expected infectivity given an exponential decay of viable Ad5 particles with characteristic lifetime τ, equation (1). (b,c) Fraction of cells infected versus storage time in different sucrose concentrations ranging from 0.14 to 1.7 M at RT (b) and at 37 °C (c). (d) The lifetime of Ad5 virus as extracted from the fits at 37 °C (main panel) and at RT (inset). (A comparison with the infectivity change for herpes simplex virus-2, another example of double-stranded DNA viruses, can be found in Supplementary Fig. 1.) The experiments were performed in duplicate. Error bars represent the s.d. d, days.
