Figure 3: Maintained Ad5 infectivity versus time in the presence of anionic NPs.
From: Additives for vaccine storage to improve thermal stability of adenoviruses from hours to months
(a,b) Fraction of cells infected versus storage time in days for Ad5 stored in presence of different concentrations of anionic MUS:OT NPs at RT (a) and at 37 °C (b). Curves are fits to the expectation for an exponential decay of viable Ad5 particles with time. (c) Characteristic lifetime τ extracted from the fits at 37 °C (main panel) and at RT (inset). (d) Fraction of cells infected versus storage time in days for Ad5 stored in presence of cationic N,N,N-trimethyl(11-mercaptoundecyl) ammonium chloride:OT NPs at a single concentration of 10−7 M in PBS at RT (blue) and at 37 °C (red) along with the fit to the expected loss of infectivity. The experiments were performed in duplicate. Error bars represent the s.d. d, days.
