Figure 4: In vivo experiments with stabilized Ad-NSP1 after 10 days of incubation in PEG or sucrose solution.
From: Additives for vaccine storage to improve thermal stability of adenoviruses from hours to months
Adenovirus vector expressing NSP1 from Chikungunya virus was incubated for 10 days at RT or at 37 °C in PBS, 0.6 M sucrose or 1 μM PEG. Mice were vaccinated by intramuscular injection of 100 μl of Ad-NSP1 from each stabilization condition (n=3 per group). At 10 days post vaccination, mouse splenocytes were analysed by interferon gamma ELIspot for the frequency of T cells responding to NSP1 peptides. Ad-NSP1 stabilized in sucrose significantly increased the frequency of T cells responding to NSP1. Photographs of the wells of ELIspot analysis can be found in Supplementary Fig. 8. Data are representative of two independent experiments. Centre values indicate the mean, and error bars represent the s.e.m. DMSO, dimethylsulphoxide; PMA, phorbol myristate acetate.
