Figure 2: Global analysis of RNAi screen.
From: Defining functional interactions during biogenesis of epithelial junctions
(a) Representative processed images of the parameters E-cad, Jun-A and Cyt-A from controls and selected RNAi samples with positive or negative Z-scores. (b) Distribution of Z-scores of the primary RNAi screen data set (Supplementary Fig. 3a). Grey line marks cutoff Z-scores (+/− 1.65). (c–f) Correlation of the Z-scores of different parameters obtained in the primary screen (c,d) or the candidate proteins data sets (e,f) and their respective R-values. Partial interdependence of the parameters E-cad and Jun-A is observed, but not Cyt-A. Black lines represent the linear regression models and curved red lines show their 95% confidence interval. (g) Distribution of candidate proteins according to their phenotype showing interference with a single (single) or multiple parameters (double, triple) following depletion of a single protein. Values show the relative percentage in each subgroup. (h) Venn diagram showing the number of proteins and their corresponding phenotypes (single or overlapping phenotypes). Scale bar, 50 μm. Data represent median Z-score values of three independent replicates for each RNAi oligo. The number of data points (thereafter N=) for b–d, 327 or e–h, 156.
