Figure 8: DCs and/or their precursors require CCR6 for IL-23-induced inflammation. | Nature Communications

Figure 8: DCs and/or their precursors require CCR6 for IL-23-induced inflammation.

From: Monocyte-derived inflammatory Langerhans cells and dermal dendritic cells mediate psoriasis-like inflammation

Figure 8

(a) Protocol for experiments using bone marrow (BM) and thymocyte (Thy) transfers. (b) Thickness of PBS- or IL-23-injected ears of transplanted mice following injections on days 1, 3 and 5. (c) Epidermal thickness of ear skin, and (d) expression of mRNAs encoding proteins as indicated versus Gapdh mRNA on day 6 in IL-23-injected ears. (e) MHC-II+ and MHC-II++ LCs per ear on day 6 from IL-23-injected ears. Each symbol represents a sample with cells from two to three ears. (f) Dermal cells per ear from IL-23-injected ears. The samples that were analysed contained cells from one or two ears. (g) Protocol for experiments using mixed bone marrow chimeras. At 12 weeks, the mice were treated per Fig. 2a. (h) Changes in ear thickness in mice transplanted with chimeric bone marrow. (i) Skin histology on day 6 of mice as in h. Scale bar, 200 μm. (j) Epidermal thickness of ear skin, and (k) expression of mRNA encoding IL-22 versus Gapdh mRNA on day 6 in IL-23-injected ears of mice as indicated by the key. The data are displayed from one experiment representative of two with a total of seven PBS-injected and nine IL-23-injected mice in each transplanted group (b; mean±s.d.; pairwise statistical comparison was done at day 6 using mice from all the experiments); or two experiments with a total of three mice per group (c); or two experiments with a total of four mice per group (d); or four experiments with a total of nine WT and 14 Ccr6−/− mice (e); or three experiments with a total of nine WT and 11 Ccr6−/− mice (f); or one experiment representative of three with a minimum of 10 mice total per group (h; mean±s.d.; pairwise statistical comparison was done at day 6 using mice from all the experiments); or one experiment representative of three (i); or three experiments with a total of 19 mice (j); or two experiments with a total of 18 mice (k). The data are presented as mean±s.e.m. unless otherwise noted. NS, not significant; *P<0.05, **P<0.01, ***P<0.001 (unpaired Student’s t-test).

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