Figure 2: sST2 inhibits the malignant growth of human CRC cells. | Nature Communications

Figure 2: sST2 inhibits the malignant growth of human CRC cells.

From: Soluble IL-33 receptor sST2 inhibits colorectal cancer malignant growth by modifying the tumour microenvironment

Figure 2

(a) qRT-PCR analysis of ST2L and sST2 mRNA expression in SW480 and SW620 cell lines. The expression levels are presented as the ratio to the expression of GAPDH (n=3). (b) RT-PCR analysis of ST2L, sST2, IL1RAP, MyD88 and IL33 mRNA expression in SW480, SW620, SW480-shCont and SW480-shsST2 cells. GAPDH served as the loading control. (c) Secretion of sST2 in the culture supernatants by SW480, SW620, SW480-shCont and SW480-shsST2 cells (n=3). (d) In vitro growth of SW480, SW620, SW480-shCont and SW480-shsST2 cells (n=3). (e) Tumour growth of SW480, SW620, SW480-shCont and SW480-shsST2 cells. The cells (1 × 106 cells) were subcutaneously implanted with 50% Matrigel into BALB/c nude mice (n=7 mice per group). (f) qPCR analysis of Alu sequence in the lungs of mice bearing SW480 (three different portions excised from a tumour), SW620 (15 different portions excised from 7 lungs), SW480-shCont (three different portions excised from a lung) and SW480-shsST2 tumours (13 different portions excised from 7 lungs). (g) Tumour angiogenesis. CD31 staining of cryostat sections of tumours formed by SW480, SW620, SW480-shCont and SW480-shsST2 cells (left) and the vessel density in the tumours (right) (n=15 fields). Scale bar: 200 μm. The data are shown as the mean±s.d. n.d. not detected. *P<0.05, **P<0.01, ***P<0.001 by Student’s t-test.

Back to article page